RANTES (CCL5) Rabbit Polyclonal Antibody

CAT#: AP20618PU-N

RANTES (CCL5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of RANTES Antibody at 1/500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: H9C2 whole cell lysate.

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  Immunohistochemistry analysis of RANTES Antibody in paraffin-embedded human lung carcinoma tissue.

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  Immunohistochemistry analysis of RANTES Antibody in paraffin-embedded human breast carcinoma tissue at 1/100.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 50-100 of Human RANTES.
• Specificity: This antibody detects endogenous levels of RANTES protein.
  (region surrounding Tyr84)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~10 kDa
• Gene Name: Homo sapiens C-C motif chemokine ligand 5 (CCL5), transcript variant 1
• Database Link:
  Entrez Gene 20304 MouseEntrez Gene 81780 RatEntrez Gene 6352 Human
• Background: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
• Synonyms: C-C motif chemokine 5, D17S136E, SCYA5, Small-inducible cytokine A5, SIS-delta, EoCP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway