PTCH1 (C-term) Goat Polyclonal Antibody

CAT#: AP22448PU-N

PTCH1 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

PTCH1 antibody staining of Paraffin Embedded Human Colon at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: Peptide ELISA: 1/8000 (Detection Limit).
  Immunohsitochemistry on Paraffin Sections: 3-6 µg/ml.
  Staining of Embedded Human Colon shows textured nuclear staining of the absorptive cells.
  Immunofluorescence: 5 µg/ml. Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membrane of NIH3T3 cells.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of Human PTCH1 (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1)
• Specificity: This antibody is expected to recognise all reported isoforms of PTCH1.
  Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens patched 1 (PTCH1), transcript variant 1b
• Database Link:
  Entrez Gene 19206 MouseEntrez Gene 89830 RatEntrez Gene 5727 Human
• Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.
• Synonyms: PTCH, PTC1, PTC, Patched 1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer