Clusterin (CLU) (488-501) Goat Polyclonal Antibody

CAT#: AP22475PU-N

Clusterin (CLU) (488-501) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.3 ug/ml) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Human Testis (formalin-fixed, paraffin-embedded) stained with CLU antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/32000, 0.3 - 1 µg/ml.
• Reactivities: Human, Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminus of human CLU/Clusterin (NP_001822.2; NP_976084.1).
• Specificity: This antibody detects Apolipoprotein J (Apo J) at C-term.
• Formulation: Tris saline buffer, pH~7.3, 0.5% BSA, 0.02% Sodium Azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens clusterin (CLU), transcript variant 1
• Database Link:
  Entrez Gene 1191 Human
• Background: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself.
• Synonyms: Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40,40

Reference Data

• Protein Families: Druggable Genome, Secreted Protein