LXR beta (NR1H2) (2-15) Goat Polyclonal Antibody

CAT#: AP22491PU-N

LXR beta (NR1H2) (2-15) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1 ug/ml) staining of human bone marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Human Spleen (formalin-fixed, paraffin-embedded) stained with NR1H2 antibody at 3 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Uterus (formalin-fixed, paraffin-embedded) stained with NR1H2 antibody at 3 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/64000.
  Immunohistochemistry on Paraffin Sections: 3 µg/ml.
  Western Blot: 1 - 3 µg/ml.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from (N-term) of human NR1H2
• Specificity: This antibody detects NR1H2 (N-term).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nuclear receptor subfamily 1 group H member 2 (NR1H2), transcript variant 1
• Database Link:
  Entrez Gene 22260 MouseEntrez Gene 58851 RatEntrez Gene 7376 Human
• Background: Liver X receptor beta (LXR beta), a NR1 thyroid hormone-like receptor, has been shown to affect cholesterol and bile acid metabolism, as well as fatty acid metabolism. LXR beta mediates gene products controlling intracellular cholesterol homeostasis through the interaction with oxysterols including 22(R)-hydroxycholesterol, 24(S)-hydroxycholesterol, and 24(S),25-epoxycholesterol. LXR beta binds as a heterodimer to target sequences with retinoid X receptors and has been shown to activate a diverse set of genes involved in cholesterol and fatty acid metabolism, including ABCA1, ABCG5, ABCG8, SREBP-1c, fatty acid synthase (FAS), and the apoE/C-I/C-IV/C-II gene cluster upon ligand activation. Recently both LXR alpha and LXR beta have been shown to be important factors in atherosclerosis (Tangirala et al. 2002).
• Synonyms: LXRB, NER, UNR

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors