BHMT (391-402) Goat Polyclonal Antibody

CAT#: AP22552PU-N

BHMT (391-402) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Kidney (formalin-fixed, paraffin-embedded) stained with BHMT antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Liver (formalin-fixed, paraffin-embedded) stained with BHMT antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/16000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.03 - 0.1 µg/ml.
• Reactivities: Hamster, Human, Monkey, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-term of human BHMT
• Specificity: This antibody detects BHMT (C-term).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens betaine--homocysteine S-methyltransferase (BHMT)
• Database Link:
  Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 635 Human
• Background: Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
• Synonyms: Betaine--homocysteine S-methyltransferase 1

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways