Apolipoprotein B (APOB) Goat Polyclonal Antibody

CAT#: AP22590PU-N

Apolipoprotein B (APOB) goat polyclonal antibody, Purified

Product Images

Human Liver (formalin-fixed, paraffin-embedded) stained with APOB antibody AP22590PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/2000 - 1/10000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/2000 - 1/10000.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: ApoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification,
• Specificity: Typically less than 1% cross reactivity againstother types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Formulation: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store vial at 2 - 8 °C prior to opening.
  Following opening store (in aliquots) at -20 °C. For extended storage mix with an equal volume of glycerol. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens apolipoprotein B (APOB)
• Database Link:
  Entrez Gene 338 Human
• Background: Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563 amino acids, and the mature apoB-100 has 4536 amino acid residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144 amino acid residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.
• Synonyms: ApoB, Apo-B, ApoB100, ApoB-100

Reference Data

• Protein Families: Druggable Genome, Transmembrane