HMGA1 (9-21) Goat Polyclonal Antibody

CAT#: AP23070PU-N

HMGA1 (9-21) goat polyclonal antibody, Aff - Purified

Product Images

WB Image of AP23070PU-N Staining (0.5ug/ml) of Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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  IHC Image of AP23070PU-N Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  IHC Image of AP23070PU-N Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/16000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
  Western Blot: 0.5 - 1 µg/ml.
• Reactivities: Bat, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: HMGA1 antibody was raised against peptide from the internal region (near N Terminus) of human HMGA1.
• Specificity: This antibody reacts to High Mobility Group Protein Hmg-i (HMGA1) at aa 9-21.
  It is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). The antibody is not expected to cross-react with HMGA2.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2
• Database Link:
  Entrez Gene 15361 MouseEntrez Gene 117062 RatEntrez Gene 3159 Human
• Background: HMGIY / HMGA1 is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins.
• Synonyms: High mobility group protein HMG-I/HMG-Y, High mobility group protein A1, High mobility group protein R

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors