Fas (N-term) Rabbit Polyclonal Antibody

CAT#: AP23251PU-N

Fas (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Lane 1: Recombinant Mouse FAS Protein 10ng.Lane 2: Recombinant Mouse FAS Protein 5ng.Lane 3: Recombinant Mouse FAS Protein 2.5ng.

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  Paraffin Rat Spleen tissue lysate stained with CD95 / FAS antibody

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  Immunocytochemical staining of HeLa Cells

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: Use at 1 µg/ml with the appropriate system to detect FAS in cells and tissues.
  Immunohistochemistry on Frozen Sections: Use at 1-2 µg/ml to detect FAS in Formalin or Acetone fixed tissues.
  Immunohistochemistry on Paraffin Sections: Use at 1-2 µg/ml to detect FAS in Formalin Fixed and Paraffin Embedded tissues. Antigen Retrieval by heat is required.
  
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of Rat FAS, different from the related Mouse sequence by seven amino acids.
• Specificity: Recognizes FAS.
  No cross reactivity with other proteins.
• Formulation: 0.9 mg NaCl, 0.2 mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 5 mg BSA
  Preservative: 0.05 mg Thimerosal and 0.05 mg Sodium Azide
• Reconstitution Method: Restore with 0.2 ml of distilled water to yield a concentration of 0.5 mg/ml.
• Concentration: 0.5 mg/ml (after reconstitution)
• Purification: Immunoaffinity Chromatography
• Gene Name: Mus musculus Fas (TNF receptor superfamily member 6) (Fas), transcript variant 1
• Database Link:
  Entrez Gene 246097 RatEntrez Gene 14102 Mouse
• Background: FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6