Nogo A (RTN4) (Isoform 1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP23267PU-N

Nogo A (RTN4) (Isoform 1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using NOGO-A antibody

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect Nogo-A in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect Nogo-A in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunocytochemistry.
  Immunprecipitation.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the C-terminal of human Nogo-A
• Specificity: This antibody detects Reticulon-4 / RTN4 Isoform 1 at C-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg Sodium Azide
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Gene Name: Homo sapiens reticulon 4 (RTN4), transcript variant 1
• Database Link:
  Entrez Gene 68585 MouseEntrez Gene 83765 RatEntrez Gene 57142 Human
• Background: Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.
• Synonyms: Foocen, Neurite outgrowth inhibitor, NOGO, Neuroendocrine-specific protein, NSP, Reticulon-5, KIAA0886

Reference Data

• Protein Families: Transmembrane