Angpt2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23297PU-N

Angpt2 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using ANG2 antibody

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  Immunohistochemical analysis of paraffin embedded Human placencta sections using ANG2 antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect ANG2 in cells and tissues.
  Immunohistochemistry on Paraffin Sections: At 1-2μg/ml to detect ANG2 in formalin fixed and paraffin embedded.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping near the C-terminal (454-468) of Human Angiopoietin-2.
• Specificity: This antibody detects Angiopoietin-2 near C-term.
  No cross reactivity with other proteins.
• Formulation: 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 5mg BSA
  Preservative: 0.05mg Thimerosal, 0.05mg Sodium Azide
• Reconstitution Method: 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunoaffinity Chromatography
• Gene Name: Mus musculus angiopoietin 2 (Angpt2)
• Database Link:
  Entrez Gene 285 HumanEntrez Gene 89805 RatEntrez Gene 11601 Mouse
• Background: Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
• Synonyms: ANG-2, ANG2, ANGPT2