Complement C3 (C3) Rabbit Polyclonal Antibody

CAT#: AP31519SU-N

Complement C3 (C3) rabbit polyclonal antibody, Serum


USD 450.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ID, IP
Recommended Dilution Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3a or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
Directions for use:
Immunoelectrophoresis: Use 2 μl human plasma or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Single Radial Immunodiffusion: Use 1 percent antiserum in the gel.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen C3a is a component of complement C3 produced by its activation. It has a molecular weight of 8,900.
The protein is isolated and purified from pooled normal Human plasma.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
Formulation No preservative added. - No foreign proteins added.
State: Serum
State: Delipidated, heat inactivated, lyophilized, stable whole serum.
Reconstitution Method Restore with 1 ml sterile distilled water
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens complement component 2 (C2), transcript variant 1
Synonyms CPAMD1, complement component 3
Note Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data
Protein Families Druggable Genome, Protease, Secreted Protein
Protein Pathways Complement and coagulation cascades, Systemic lupus erythematosus

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.