Alpha 2 Antiplasmin (SERPINF2) Rabbit Polyclonal Antibody

CAT#: AP31528SU-N

Alpha 2 Antiplasmin (SERPINF2) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.
  Directions for use: In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2% depending on the test arrangement.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Alpha-2 antiplasmin has been isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody reactivity is restricted to alpha-2 antiplasmin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen.
  Cross-reactivity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: No preservative added. - No foreign proteins added.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole serum.
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2 (SERPINF2), transcript variant 1
• Database Link:
  Entrez Gene 5345 Human
• Background: Alpha-2 antiplasmin is a single-chain glycoprotein with a molecular weight of about 65,000.The normal concentration in human plasma is 60 μg/ml. Platelets contain significant amounts of the protein, which is released along with platelet factor 4, following thrombin stimulation. It can form 1:1 complexes with circulating plasmin. Alpha-2 antiplasmin is the most important inhibitor of plasmin-mediated fibrinolysis, but also capable of inactivating factor XIIa, kallikrein, factor XIa, thrombin and factor Xa. The importance of the alpha-2 antiplasmin as regulator of the fibrinolytic system was detected by the discovery of a congenital deficiency.
• Synonyms: Alpha-2-AP, AAP, PLI, Alpha-2-PI, Serpin F2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades