NFATC4 Goat Polyclonal Antibody

CAT#: AP31861PU-N

NFATC4 goat polyclonal antibody, Aff - Purified

Product Images

Staining of paraffin embedded Human Placenta using NFATC4 Antibody. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/16000 (Detection Limit).
  Western blot: No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, Human Brain and Human Heart lysates at upto 2 µg/ml.
  Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Placenta shows weak nuclear staining in trophoblasts.
• Reactivities: Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_004545.2, NP_001185894.1, NP_001185895.1, NP_001185896.1.
• Specificity: This antibody is expected to recognise isoform 2 (NP_004545.2), Isoform 3 (NP_001185894.1), Isofrom 4 (NP_001185895.1) and Isofrom 5 (NP_001185896.1).
• Formulation: Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 1
• Database Link:
  Entrez Gene 73181 MouseEntrez Gene 305897 RatEntrez Gene 4776 Human
• Background: The protein is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a pre-existing cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4.
• Synonyms: NF-ATc4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway