CIRBP (C-term) Rabbit Polyclonal Antibody

CAT#: AP50926PU-N

CIRBP (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CIRBP Antibody (C-term) in Y79 cell line lysates (35ug/lane). CIRBP (arrow) was detected using the purified Pab.

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  Western blot analysis of CIRBP (arrow) using rabbit polyclonal CIRBP Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CIRBP gene.

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  CIRBP Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CIRBP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1000.
  Western blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 145-172 amino acids from the C-terminal region of human CIRBP
• Specificity: This antibody reacts to CIRBP.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 18648 Da
• Gene Name: Homo sapiens cold inducible RNA binding protein (CIRBP), transcript variant 1
• Database Link:
  Entrez Gene 1153 Human
• Background: CIRBP is cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. It acts as a translational activator and seems to play an essential role in cold-induced suppression of cell proliferation. It binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. This protein acts as a translational repressor (By similarity) and promotes assembly of stress granules (SGs), when overexpressed.
• Synonyms: A18HNRNP, CIRP