EFEMP1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP51374PU-N

EFEMP1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Fibulin-3 (arrow) in mouse lung tissue lysates (35ug/lane) using Fibulin-3 antibody. (N-term).

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  Immunohistochemistry analysis in human lung carcinoma (Formalin-fixed, Paraffin-embedded) using Fibulin-3 antibody. (N-term), followed by peroxidase-conjugated secondary antibodyand DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using Fibulin-3 antibody. (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 126-156aa) of human Fibulin-3
• Specificity: This antibody recognizes Fibulin-3 at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: EGF containing fibulin like extracellular matrix protein 1
• Database Link:
  Entrez Gene 216616 MouseEntrez Gene 2202 Human
  UniProt ID Q12805
• Background: EFEMP1 encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, Fibulin-3 contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors.
• Synonyms: DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, Fibulin 3, EFEMP1, FBLN3
• Note: Molecular Weight: 54641 Da

Reference Data

• Protein Families: Secreted Protein