IGSF8 (Center) Rabbit Polyclonal Antibody

CAT#: AP52177PU-N

IGSF8 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse cerebellum tissue lysates (35ug/lane) using Cd316 / IGSF8 antibody. This demonstrates the IGSF8 antibody detected the IGSF8 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded (FFPE) human kidney tissue using CD316 / IGSF8 antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGSF8 antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry (Paraffin sections): 1/10-1/50
  Western blotting: 1/100-1/500
  ELISA: 1/1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (233-262 aa) - KLH conjugated - corresponding to internal domain of human IGSF8.
• Specificity: This antibody detects IGSF8 (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens immunoglobulin superfamily, member 8 (IGSF8), transcript variant 2
• Database Link:
  Entrez Gene 140559 MouseEntrez Gene 93185 Human
• Background: GSF8/PGRL/EWI-2 is a membrane protein that contains 4 Ig domains and forms highly proximal, specific and stoichiometric complexes with both CD9 and CD81 a tetraspanin. Tetraspanins and their molecular partners from primary complexes that then undergo tetraspanin-tetraspanin interactions to form a network of molecular interactions call a tetraspanin web. IGSF8 then may play a key role in diverse functions ascribed to CD81 and CD9 including oocyte fertilization or hepatitis C virus function, regulation of proliferation and differentiation of keratinocytes. It may also be a negative regulator of cell motility and suppress T-cell mobility coordinately with CD81.
• Synonyms: CD81 partner 3, LIR-D1, CD81P3, EWI2, KCT4
• Note: Molecular Weight: 65034 Da

Reference Data

• Protein Families: Transmembrane