IL17B (Center) Rabbit Polyclonal Antibody

CAT#: AP52186PU-N

IL17B (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IL17B (arrow) using Interleukin-17B / IL17B antibody. ; 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL17B gene.

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  Western blot analysis in mouse spleen tissue lysates (35ug/lane) using Interleukin-17B / IL17B antibody. This demonstrates the IL17B antibody detected the IL17B protein (arrow).

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  Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using Interleukin-17B / IL17B antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL17B antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of IL17B with Hela cells using Interleukin-17B / IL17B antibody., followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 46-74aa) of human Interleukin-17B / IL17B
• Specificity: This antibody recognizes Interleukin-17B / IL17B.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens interleukin 17B (IL17B), transcript variant 1
• Database Link:
  Entrez Gene 56069 MouseEntrez Gene 27190 Human
• Background: The protein encoded by the IL17B gene is a T cell-derived cytokine that shares sequence similarity with IL17. Interleukin-17B was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
• Synonyms: IL-17B, IL20, NIRF, ZCYTO7, Neuronal interleukin-17-related factor, Interleukin-20
• Note: Molecular Weight: 20437 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction