IREB2 (Center) Rabbit Polyclonal Antibody

CAT#: AP52232PU-N

IREB2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IREB2 Antibody (Center) in Hela cell line lysates (35ug/lane). This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with IREB2 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of Hela cells using IREB2 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human IREB2
• Specificity: This antibody recognizes Human IREB2 (201-230).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens iron responsive element binding protein 2 (IREB2), transcript variant 1
• Database Link:
  Entrez Gene 3658 Human
• Background: IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
• Synonyms: IRE-BP2, Iron regulatory protein 2, IRP2
• Note: Molecular Weight: 105045 Da

Reference Data

• Protein Families: Druggable Genome