MCM6 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52640PU-N

MCM6 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MCM6 Antibody (N-term) in 293 cell line lysates (35ug/lane). This demonstrates the MCM6 antibody detected the MCM6 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue reacted with MCM6 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of Human MCM6.
• Specificity: This antibody recognizes Human MCM6 (N-term). Other species not tested.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens minichromosome maintenance complex component 6 (MCM6)
• Database Link:
  Entrez Gene 4175 Human
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
• Synonyms: Mis5, P105MCM
• Note: Molecular Weight: 92889 Da

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication