PEG10 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53253PU-N

PEG10 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PEG10 Antibody (N-term) in U251 cell line lysates (35ug/lane). This demonstrates the PEG10 antibody detected the PEG10 protein (arrow).

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  Flow cytometric analysis of U251 cells using PEG10 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEG10
• Specificity: This antibody recognizes Human PEG10 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens paternally expressed 10 (PEG10), transcript variant 1
• Database Link:
  Entrez Gene 23089 Human
• Background: This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas.
• Synonyms: EDR, KIAA1051, MAR2, MART2, MEF3L1, MEF3-like protein 1, RGAG3
• Note: Molecular Weight: 80173 Da