RACGAP1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53556PU-N

RACGAP1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of RACGAP1 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow

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  Formalin fixed, paraffin embedded human testis tissue stained with RACGAP1 Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  Flow Cytometric analysis of MCF-7 cells using RACGAP1 Antibody (N-term) (right histogram) compared to a Negative Control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of Human RACGAP1
• Specificity: This antibody recognizes Human RACGAP1 (N-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens Rac GTPase activating protein 1 (RACGAP1), transcript variant 2
• Database Link:
  Entrez Gene 29127 Human
• Background: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
• Synonyms: MGCRACGAP, KIAA1478
• Note: Molecular Weight: 71027 Da

Reference Data

• Protein Families: Druggable Genome