RAD54 (RAD54L) (C-term) Rabbit Polyclonal Antibody

CAT#: AP53565PU-N

RAD54 (RAD54L) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of RAD54L Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). This demonstrates the RAD54 antibody detected the RAD54 protein (arrow)

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  Flow cytometric analysis of NCI-H460 cells using RAD54L Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 641-671 amino acids from the C-terminal region of Human RAD54
• Specificity: This antibody recognizes Human RAD54L (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2
• Database Link:
  Entrez Gene 8438 Human
• Background: The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.
• Synonyms: RAD54A, RAD54 homolog, hRAD54, hHR54
• Note: Molecular Weight: 84352 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination