Neuroserpin (SERPINI1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP53865PU-N

Neuroserpin (SERPINI1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis using SERPINI1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). This demonstrates the SERPINI1 antibody detected the SERPINI1 protein (arrow).

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human brain tissue stained with SERPINI1 antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINI1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal Immunofluorescent analysis of SERPINI1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 26-53 amino acids from the N-terminal region of Human SERPINI1.
• Specificity: Recognizes SERPINI1 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens serpin family I member 1 (SERPINI1), transcript variant 2
• Database Link:
  Entrez Gene 5274 Human
• Background: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Synonyms: Serpin I1, PI12, PI-12
• Note: Molecular Weight: 46427 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein