Squalene Epoxidase (SQLE) (N-term) Rabbit Polyclonal Antibody

CAT#: AP54036PU-N

Squalene Epoxidase (SQLE) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in A549 cell line lysates (35ug/lane) using Squalene epoxidase / SQLE antibody. (N-term). This demonstrates the SQLE antibody detected the SQLE protein (arrow).

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  Immunohistochemistry analysis in human testis tissue (Formalin-fixed, Paraffin-embedded) using Squalene epoxidase / SQLE antibody. (N-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SQLE antibody for IHC; Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 67-97aa) of human Squalene epoxidase / SQLE
• Specificity: This antibody recognizes Squalene epoxidase / SQLE at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens squalene epoxidase (SQLE)
• Database Link:
  Entrez Gene 6713 Human
• Background: Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway.
• Synonyms: Squalene monooxygenase, ERG1
• Note: Molecular Weight: 63923 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis