UBF1 (UBTF) (C-term) Rabbit Polyclonal Antibody

CAT#: AP54449PU-N

UBF1 (UBTF) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

UBTF Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the UBTF antibody detected the UBTF protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 630-660 amino acids from the C-terminal region of human UBTF
• Specificity: This antibody detects UBTF / UBF (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 2
• Database Link:
  Entrez Gene 7343 Human
• Background: Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).
• Synonyms: Nucleolar transcription factor 1, Upstream-binding factor 1, UBF1, UBF-1, Autoantigen NOR-90
• Note: Molecular Weight: 89406 Da

Reference Data

• Protein Families: Transcription Factors