VPS52 (C-term) Rabbit Polyclonal Antibody

CAT#: AP54517PU-N

VPS52 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

VPS52 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the VPS52 antibody detected the VPS52 protein (arrow).

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  VPS52 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VPS52 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of VPS52 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  VPS52 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Immunohistochemistry on paraffin sections: 1:50~100.
  Flow cytometry: 1:10~50.
  Immunofluorescence: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 613-641 amino acids from the C-terminal region of human VPS52
• Specificity: This antibody detects VPS52 (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens vacuolar protein sorting 52 homolog (S. cerevisiae) (VPS52), transcript variant 1
• Database Link:
  Entrez Gene 6293 Human
• Background: This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
• Synonyms: SACM2L
• Note: Molecular Weight: 82221 Da