ZP4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP54737PU-N

ZP4 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1;000.
  Western blot: 1:100~500.
  Immunohistochemistry on paraffin sections: 1:10~50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human ZP4
• Specificity: This antibody detects ZP4 / ZPB (C-term).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide affinity purification
• Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens zona pellucida glycoprotein 4 (ZP4)
• Database Link:
  Entrez Gene 57829 Human
• Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.
• Synonyms: ZBP; ZP1; ZPB
• Note: Molecular Weight: 59400 Da

Reference Data

• Protein Families: Secreted Protein, Transmembrane