Col5a1 Rabbit Polyclonal Antibody

CAT#: BP2208

Col5a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Suitable for :
  ELISA (dilution >1:100-1:200).
  IFA with fluorescein anti-rabbit IgG conjugate (didilution at >1:40 on frozen rat tissue sections).
  Immunostaining of extra or intracellular components in light microscopy.
  Quantitation of Collagen Type V in biological fluids or in culture supernatants.
  (SP)RIA.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Collagen type V extracted and purified from rat skin.
• Specificity: Cross-Reactivity (% at 1:10 RIA dilution)
  Rat Collagen Type V 100%
  Rat Collagen Types I, III < 0.1%
  Rat Elastin < 0.1%
• Formulation: State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Reconstitute with 0.5 ml deionized water.
• Purification: Ion exchange chromatography (DEAE-Trisacryl).
• Storage: Lyophilized store the antibody at 2-8°C. Following reconstitution store at -20 °C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: Rattus norvegicus collagen type V alpha 1 chain (Col5a1)
• Database Link:
  Entrez Gene 85490 Rat
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.
  Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
• Synonyms: COL5A1