Col1a1 Rabbit Polyclonal Antibody

CAT#: BP8003

Col1a1 rabbit polyclonal antibody, Purified


USD 1,050.00

2 Weeks*

Size
    • 500 ul

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Specifications

Product Data
Applications ELISA, IF, IHC, R
Recommended Dilution Western Blot.
Radioimmunoassay:
1/200.
ELISA:
≥ 1/200.
Immunfluorescence (Indirect):  ≥ 1/80.
Immunohistochemistry on Paraffin Sections:  ≥ 1/500. 
Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. 
Incubation time: 60 min at RT or 2-8°C over night. 
Positive Control: Mouse skin.
Reactivities Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Purified Collagen type I from Mouse skin
Specificity This antibody reacts with Mouse Collagen type I (100%), Mouse Collagen type II and IV (< 0.1%), Mouse Collage type III (< 1%), Human, Chicken and Rat Collagen type I (< 0.1%, RIA at 1/200 dilution).
Formulation Phosphate Buffered Solution without stabilizer or preservatives
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 0.1 ml sterile distilled water. For further dilution use appropriate antibody diluent.
Concentration 1.0 mg/ml
Purification Affinity Chromatography
Gene Name Mus musculus collagen, type I, alpha 1 (Col1a1)
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

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