Col1a1 Rabbit Polyclonal Antibody

CAT#: BP8003

Col1a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: Western Blot.
  Radioimmunoassay: 1/200.
  ELISA: ≥ 1/200.
  Immunfluorescence (Indirect): ≥ 1/80.
  Immunohistochemistry on Paraffin Sections: ≥ 1/500.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Incubation time: 60 min at RT or 2-8°C over night.
  Positive Control: Mouse skin.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type I from Mouse skin
• Specificity: This antibody reacts with Mouse Collagen type I (100%), Mouse Collagen type II and IV (< 0.1%), Mouse Collage type III (< 1%), Human, Chicken and Rat Collagen type I (< 0.1%, RIA at 1/200 dilution).
• Formulation: Phosphate Buffered Solution without stabilizer or preservatives
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.1 ml sterile distilled water. For further dilution use appropriate antibody diluent.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography
• Gene Name: Mus musculus collagen, type I, alpha 1 (Col1a1)
• Database Link:
  Entrez Gene 12842 Mouse
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen