BrdU Rat Monoclonal Antibody [Clone ID: OTI5E6]

CAT#: CF190094

Carrier-free (BSA/glycerol-free) BrdU rat monoclonal antibody, clone OTI5E6 (formerly 5E6)

Product Images

Immunohistochemical staining of paraffin-embedded colon tissue from BrdU injected mouse using anti-BrdU rat monoclonal antibody (TA190094). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded colon tissue from IDU injected mouse using anti-BrdU rat monoclonal antibody (TA190094). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded colon tissue from ClDU injected mouse using anti-BrdU rat monoclonal antibody (TA190094). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI5E6
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.