YAP1 Mouse Monoclonal Antibody [Clone ID: OTI5E6]

CAT#: CF813309

Carrier-free (BSA/glycerol-free) YAP1 mouse monoclonal antibody,clone OTI5E6

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and YAP1-Knockout HeLa cells (KO, Cat# LC832485) were separated by SDS-PAGE and immunoblotted with anti-YAP1 monoclonal antibody TA813309 (1:1000). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-YAP1 monoclonal antibody (1:1000).

Specifications

Product Data

• Clone Name: OTI5E6
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 347-504 of human YAP1 (NP_001123617) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.3 kDa
• Gene Name: Homo sapiens Yes associated protein 1 (YAP1), transcript variant 1, mRNA.
• Database Link:
  NP_001123617
  Entrez Gene 22601 Mouse
• Background: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
• Synonyms: COB1; YAP; YAP2; YAP65; YKI

Reference Data

• Protein Families: Druggable Genome