Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: OTI5D1]

CAT#: CF500018

Carrier-free (BSA/glycerol-free) CK19 mouse monoclonal antibody, clone OTI5D1 (formerly 5D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (RC209707, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19. Positive lysates LY419428 (100ug) and LC419428 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-KRT19 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500018)

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  Anti-KRT19 mouse monoclonal antibody (TA500018) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT19 (RC209707).

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  Immunofluorescent staining of HeLa cells using anti-KRT19 mouse monoclonal antibody (TA500018).

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Specifications

Product Data

• Clone Name: OTI5D1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:10000~20000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 240-390 of human CK19 (NP_002267) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens keratin 19 (KRT19), mRNA.
• Database Link:
  NP_002267
  Entrez Gene 100685481 DogEntrez Gene 3880 Human
  UniProt ID P08727
• Background: Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
• Synonyms: CK19; K1CS; K19