FGF1 Mouse Monoclonal Antibody [Clone ID: OTI4D2]

CAT#: CF500064

Carrier-free (BSA/glycerol-free) aFGF mouse monoclonal antibody, clone OTI4D2 (formerly 4D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFGF (RC207434, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFGF. Positive lysates LY400278 (100ug) and LC400278 (20ug) can be purchased separately from OriGene.

• 

  aFGF antibody ( 4D2 ) at 1:50000 dilution + recombinant Human aFGF (Cat# RC207434)

• 

  Anti-AFGF mouse monoclonal antibody (TA500064) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFGF (RC207434).

Specifications

Product Data

• Clone Name: OTI4D2
• Applications: IF, WB
• Recommended Dilution: WB 1:5000~50000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.8 kDa
• Gene Name: Homo sapiens fibroblast growth factor 1 (FGF1), transcript variant 1, mRNA.
• Database Link:
  NP_000791
  Entrez Gene 14164 MouseEntrez Gene 25317 RatEntrez Gene 2246 Human
  UniProt ID P05230
• Background: FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described.
• Synonyms: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton