FGF2 Mouse Monoclonal Antibody [Clone ID: OTI3D10]

CAT#: CF500269

Carrier-free (BSA/glycerol-free) BFGF mouse monoclonal antibody, clone OTI3D10 (formerly 3D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF. Positive lysates LY400733 (100ug) and LC400733 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC217426 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BFGF antibody (TA500269), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3D10
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: Homo sapiens fibroblast growth factor 2 (FGF2), mRNA.
• Database Link:
  NP_001997
  Entrez Gene 14173 MouseEntrez Gene 54250 RatEntrez Gene 2247 Human
  UniProt ID P09038
• Background: BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Synonyms: BFGF; FGF-2; FGFB; HBGF-2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton