STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10]

CAT#: CF500408

Carrier-free (BSA/glycerol-free) STK3 mouse monoclonal antibody, clone OTI4G10 (formerly 4G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK3 (RC203897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK3. Positive lysates LY401892 (100ug) and LC401892 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-STK3 monoclonal antibody.

• 

  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-STK3 monoclonal antibody (1:200).

• 

  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-STK3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

• 

  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-STK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500408, Dilution 1:50)

Show all 13 images

Specifications

Product Data

• Clone Name: OTI4G10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000; IHC 1:50
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human STK3 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.3 kDa
• Gene Name: Homo sapiens serine/threonine kinase 3 (STK3), transcript variant 1, mRNA.
• Database Link:
  NP_006272
  Entrez Gene 56274 MouseEntrez Gene 65189 RatEntrez Gene 475045 DogEntrez Gene 707425 MonkeyEntrez Gene 6788 Human
  UniProt ID Q13188
• Background: Protein kinase activation is a frequent response of cells to treatment with growth factors,chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast ' sterile 20'(Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand
• Synonyms: KRS1; MST2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway