LAT2 (SLC7A8) Mouse Monoclonal Antibody [Clone ID: OTI4H10]

CAT#: CF500632

Carrier-free (BSA/glycerol-free) SLC7A8 mouse monoclonal antibody, clone OTI4H10 (formerly 4H10)

Product Images

Anti-SLC7A8 mouse monoclonal antibody (TA500632) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8 (RC208586).

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  Immunofluorescent staining of HepG2 cells using anti-SLC7A8 mouse monoclonal antibody (TA500632).

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  Immunoprecipitation (IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  Flow cytometric Analysis of Jurkat cells, using anti-SLC7A8 antibody (TA500632), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI4H10
• Applications: FC, IF, IP
• Recommended Dilution: IF 1:50~100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLC7A8 (NP_036376) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.4 kDa
• Gene Name: Homo sapiens solute carrier family 7 member 8 (SLC7A8), transcript variant 1, mRNA.
• Database Link:
  NP_036376
  Entrez Gene 50934 MouseEntrez Gene 84551 RatEntrez Gene 23428 Human
  UniProt ID Q9UHI5
• Background: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney
• Synonyms: LAT2; LPI-PC1

Reference Data

• Protein Families: Druggable Genome, Transmembrane