FKBP51 (FKBP5) Mouse Monoclonal Antibody [Clone ID: OTI3E9]

CAT#: CF500673

Carrier-free (BSA/glycerol-free) FKBP5 mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP5 (RC210608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP5. Positive lysates LY401331 (100ug) and LC401331 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-FKBP5 monoclonal antibody (1:200).

• 

  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-FKBP5 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FKBP5-Knockout HeLa cells (KO, Cat# [LC832231]) were separated by SDS-PAGE and immunoblotted with anti-FKBP5 monoclonal antibody TA500673 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

• 

  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-FKBP5mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500673, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-FKBP5mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500673, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FKBP5mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500673, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-FKBP5mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500673, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-FKBP5mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500673, Dilution 1:50)

• Anti-FKBP5 mouse monoclonal antibody (TA500673) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FKBP5 (RC210608).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI3E9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human FKBP5 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.2 kDa
• Gene Name: Homo sapiens FKBP prolyl isomerase 5 (FKBP5), transcript variant 1, mRNA.
• Database Link:
  NP_004108
  Entrez Gene 14229 MouseEntrez Gene 361810 RatEntrez Gene 2289 Human
  UniProt ID Q13451
• Background: The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition.It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.
• Synonyms: AIG6; FKBP51; FKBP54; P54; PPIase; Ptg-10

Reference Data

• Protein Families: Druggable Genome