PFKFB4 Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CAT#: CF500809

Carrier-free (BSA/glycerol-free) PFKFB4 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKFB4 (RC201573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKFB4. Positive lysates LY401448 (100ug) and LC401448 (20ug) can be purchased separately from OriGene.

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  Anti-PFKFB4 mouse monoclonal antibody (TA500809) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKFB4 (RC201573).

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  Immunofluorescent staining of HepG2 cells using anti-PFKFB4 mouse monoclonal antibody (TA500809).

Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PFKFB4 (NP_004558) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.0 kDa
• Gene Name: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), transcript variant 2, mRNA.
• Database Link:
  NP_004558
  Entrez Gene 54283 RatEntrez Gene 270198 MouseEntrez Gene 5210 Human
  UniProt ID Q16877
• Background: Synthesis and degradation of fructose 2,6-bisphosphate
• Synonyms: 6-biphosphatase-4; 6-biphosphatase 4; 6-phosphofructo-2-kinase; bifunctional enzyme with kinase and biphosphatase activities; fructose-2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism