BHMT Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CAT#: CF500961

Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT. Positive lysates LY400644 (100ug) and LC400644 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500961, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500961, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500961, Dilution 1:50)

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  HEK293T cells transfected with either pCMV6-ENTRY BHMT (RC203148) (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal (TA500961), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1E5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: Homo sapiens betaine--homocysteine S-methyltransferase (BHMT), mRNA.
• Database Link:
  NP_001704
  Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 635 Human
  UniProt ID Q93088
• Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
• Synonyms: BHMT1; HEL-S-61p

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways