SIL1 Mouse Monoclonal Antibody [Clone ID: OTI3B11]

CAT#: CF501010

Carrier-free (BSA/glycerol-free) SIL1 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (RC211850, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1. Positive lysates LY402924 (100ug) and LC402924 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded lymphoma tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501010, Dilution 1:50)

• Anti-SIL1 mouse monoclonal antibody (TA501010) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1 (RC211850).

• HEK293T cells transfected with either pCMV6-ENTRY SIL1 (RC211850) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SIL1 mouse monoclonal (TA501010), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3B11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.1 kDa
• Gene Name: Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.
• Database Link:
  NP_071909
  Entrez Gene 291673 RatEntrez Gene 64374 Human
  UniProt ID Q9H173
• Background: This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.
• Synonyms: BAP; MSS; ULG5

Reference Data

• Protein Families: Protease, Secreted Protein, Transmembrane