KIAA1576 (VAT1L) Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF501107

Carrier-free (BSA/glycerol-free) VAT1L mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAT1L (RC207266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAT1L. Positive lysates LY412225 (100ug) and LC412225 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-VAT1L monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-VAT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501107, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-VAT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501107, Dilution 1:50)

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  Anti-VAT1L mouse monoclonal antibody (TA501107) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VAT1L (RC207266).

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  HEK293T cells transfected with either pCMV6-ENTRY VAT1L (RC207266) (Red) or empty vector control plasmid (Blue) were immunostained with anti-VAT1L mouse monoclonal (TA501107), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VAT1L (NP_065978) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.7 kDa
• Gene Name: Homo sapiens vesicle amine transport 1 like (VAT1L), mRNA.
• Database Link:
  NP_065978
  Entrez Gene 270097 MouseEntrez Gene 57687 Human
  UniProt ID Q9HCJ6
• Synonyms: KIAA1576

Reference Data

• Protein Families: Druggable Genome