EMG1 Mouse Monoclonal Antibody [Clone ID: OTI1B8]

CAT#: CF501209

Carrier-free (BSA/glycerol-free) EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (RC208885, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EMG1. Positive lysates LY401906 (100ug) and LC401906 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.

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  Anti-EMG1 mouse monoclonal antibody (TA501209) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1 (RC208885 ).

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  Flow cytometric Analysis of Hela cells, using anti-EMG1 antibody (TA501209), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-EMG1 antibody (TA501209), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.5 kDa
• Gene Name: Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.
• Database Link:
  NP_006322
  Entrez Gene 14791 MouseEntrez Gene 312706 RatEntrez Gene 477708 DogEntrez Gene 722122 MonkeyEntrez Gene 10436 Human
  UniProt ID Q92979
• Background: This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. COMPLETENESS: complete on the 3' end.
• Synonyms: C2F; Grcc2f; NEP1