BIRC5 Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CAT#: CF501245

Carrier-free (BSA/glycerol-free) BIRC5/Survivin mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5. Positive lysates LY400469 (100ug) and LC400469 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BIRC5 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA501245, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA501245, Dilution 1:50)

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY BIRC5 (RC205935) using anti-BIRC5 antibody (TA501245/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

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  HEK293T cells transfected with either RC205935 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BIRC5 antibody (TA501245), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2D9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.2 kDa
• Gene Name: Homo sapiens baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, mRNA.
• Database Link:
  NP_001159
  Entrez Gene 332 Human
  UniProt ID O15392
• Background: This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
• Synonyms: API4; EPR-1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Pathways in cancer