PEX5 Mouse Monoclonal Antibody [Clone ID: OTI6E9]

CAT#: CF501430

Carrier-free (BSA/glycerol-free) PEX5 mouse monoclonal antibody, clone OTI6E9 (formerly 6E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEX5 (RC202062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEX5. Positive lysates LY424800 (100ug) and LC424800 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PEX5 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PEX5-Knockout 293T cells (KO, Cat# LC812441) were separated by SDS-PAGE and immunoblotted with anti-PEX5 monoclonal antibody TA501430, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Anti-PEX5 mouse monoclonal antibody (TA501430) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PEX5 (RC202062).

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  HEK293T cells transfected with either RC202062 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PEX5 antibody (TA501430), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6E9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PEX5 (NP_000310) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.7 kDa
• Gene Name: Homo sapiens peroxisomal biogenesis factor 5 (PEX5), transcript variant 2, mRNA.
• Database Link:
  NP_000310
  Entrez Gene 19305 MouseEntrez Gene 312703 RatEntrez Gene 486710 DogEntrez Gene 715173 MonkeyEntrez Gene 5830 Human
  UniProt ID P50542
• Background: The product of this gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: PBD2A; PBD2B; PTS1-BP; PTS1R; PXR1; RCDP5

Reference Data

• Protein Families: Druggable Genome