PAICS Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF501470

Carrier-free (BSA/glycerol-free) PAICS mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (RC215225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS. Positive lysates LY416636 (100ug) and LC416636 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PAICS monoclonal antibody at 1:200 dilution.

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  Anti-PAICS mouse monoclonal antibody (TA501470) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS (RC215225).

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  HEK293T cells transfected with either RC215225 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody (TA501470), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-PAICS antibody (TA501470), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1B4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: Homo sapiens phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase (PAICS), transcript variant 2, mRNA.
• Database Link:
  NP_006443
  Entrez Gene 67054 MouseEntrez Gene 140946 RatEntrez Gene 10606 Human
  UniProt ID P22234
• Background: This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ADE2; ADE2H1; AIRC; PAIS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism