PRPSAP2 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF501563

Carrier-free (BSA/glycerol-free) PRPSAP2 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPSAP2 (RC210719, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPSAP2. Positive lysates LY400988 (100ug) and LC400988 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501563)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501563)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PRPSAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501563)

• 

  Anti-PRPSAP2 mouse monoclonal antibody (TA501563) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRPSAP2 (RC210719).

• 

  HEK293T cells transfected with either RC210719 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRPSAP2 antibody (TA501563), and then analyzed by flow cytometry.

Show all 6 images

Specifications

Product Data

• Clone Name: OTI1E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRPSAP2 (NP_002758) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.7 kDa
• Gene Name: Homo sapiens phosphoribosyl pyrophosphate synthetase associated protein 2 (PRPSAP2), transcript variant 1, mRNA.
• Database Link:
  NP_002758
  Entrez Gene 117272 RatEntrez Gene 212627 MouseEntrez Gene 5636 Human
  UniProt ID O60256
• Background: The enzyme phosphoribosylpyrophosphate synthetase (PRS) catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: PAP41

Reference Data

• Protein Families: Druggable Genome