NEK11 Mouse Monoclonal Antibody [Clone ID: OTI5E9]

CAT#: CF501608

Carrier-free (BSA/glycerol-free) NEK11 mouse monoclonal antibody, clone OTI5E9 (formerly 5E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11. Positive lysates LY411057 (100ug) and LC411057 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC221953 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK11 antibody (TA501608), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E9
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NEK11 (NP_079076) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.0 kDa
• Gene Name: Homo sapiens NIMA related kinase 11 (NEK11), transcript variant 1, mRNA.
• Database Link:
  NP_079076
  Entrez Gene 79858 Human
  UniProt ID Q8NG66
• Background: This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.
• Synonyms: FLJ23495

Reference Data

• Protein Families: Druggable Genome, Protein Kinase