PBP (PEBP1) Mouse Monoclonal Antibody [Clone ID: OTI2D4]

CAT#: CF501616

Carrier-free (BSA/glycerol-free) PEBP1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEBP1 (RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1. Positive lysates LY400913 (100ug) and LC400913 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PEBP1 monoclonal antibody.

Specifications

Product Data

• Clone Name: OTI2D4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PEBP1 (NP_002558) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.0 kDa
• Gene Name: Homo sapiens phosphatidylethanolamine binding protein 1 (PEBP1), mRNA.
• Database Link:
  NP_002558
  Entrez Gene 23980 MouseEntrez Gene 5037 Human
  UniProt ID P30086
• Background: Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin,tissue type plasminogen activator and elastase
• Synonyms: HCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; PBP; PEBP; PEBP-1; RKIP