SULT1A1 Mouse Monoclonal Antibody [Clone ID: OTI9D10]

CAT#: CF501909

Carrier-free (BSA/glycerol-free) SULT1A1 mouse monoclonal antibody, clone OTI9D10 (formerly 9D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A1 (RC201601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A1. Positive lysates LY420735 (100ug) and LC420735 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SULT1A1 monoclonal antibody.

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  Anti-SULT1A1 mouse monoclonal antibody (TA501909) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT1A1 (RC201601 ).

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  HEK293T cells transfected with either RC201601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SULT1A1 antibody (TA501909), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI9D10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SULT1A1 (NP_001046) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.0 kDa
• Gene Name: Homo sapiens sulfotransferase family 1A member 1 (SULT1A1), transcript variant 1, mRNA.
• Database Link:
  NP_001046
  Entrez Gene 6817 Human
  UniProt ID P50225
• Background: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: HAST1; HAST2; P-PST; PST; ST1A1; ST1A3; STP; STP1; TSPST1

Reference Data

• Protein Pathways: Sulfur metabolism