TTL Mouse Monoclonal Antibody [Clone ID: OTI3G5]

CAT#: CF502027

Carrier-free (BSA/glycerol-free) TTL mouse monoclonal antibody, clone OTI3G5 (formerly 3G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTL (RC207805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTL. Positive lysates LY403517 (100ug) and LC403517 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3G5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTL (NP_714923) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.0 kDa
• Gene Name: Homo sapiens tubulin tyrosine ligase (TTL), mRNA.
• Database Link:
  NP_714923
  Entrez Gene 69737 MouseEntrez Gene 171572 RatEntrez Gene 150465 Human
  UniProt ID Q8NG68
• Background: TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]). [supplied by OMIM]. COMPLETENESS: complete on the 3' end.
• Synonyms: MGC46235